PCR amplification attempted with a 384-bp megaprimer (double-stranded PCR product) paired with a 43-mer oligonucleotide primer BtV5.
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Site directed mutagenesis using double stranded megaprimers. A standard PCR using Pfu DNA polymerase was performed in two separate tubes to produce double ...
Missing: attempted- 384- paired_fig2_15065630
Aug 22, 2018 · Briefly, deletion alleles were generated using PCR amplicons containing the 1.2 kb upstream and downstream DNA regions for each rpf gene ...
Missing: 384- paired_fig2_15065630
The strategies require two rounds of PCR amplification using two flanking primers and ... The second PCR product (1837 bp) having desired mutation was purified ...
Missing: researchgate. attempted- 384- paired_fig2_15065630
This method involves serial PCR reactions to first produce an oligonucleotide containing the desired mutations and then use this "megaprimer" to introduce those ...
Missing: 384- paired_fig2_15065630
2. Run the product obtained from the PCR on an appropriate agarose gel. ... containing the megaprimer, and purify the DNA (see Note 4). ... reaction is primer ...
Missing: attempted- 384- paired_fig2_15065630
The PCR product from this reaction, the megaprimer, was then used in a second round of PCR amplification to create a larger PCR product, with the desired ...
Missing: attempted- 384- paired_fig2_15065630
PCR is an in vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short ...
Missing: 384- megaprimer- paired_fig2_15065630
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